Figure 1. Dominant Cx26 mutants form gap junction plaques.
These are deconvolved images of bulk-selected HeLa cells that express wild type human Cx26 (Cx26WT) or the indicated Cx26 mutant. The cells were labeled with a rabbit antiserum against the C-terminus of Cx26 (red) and counterstained with DAPI (blue). All cell lines had gap junction plaques at the cell borders; the R75W mutant had smaller plaques and more intracellular staining than the other mutants. Scale bar: 10 μm.