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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: FEMS Immunol Med Microbiol. 2009 Nov 23;58(2):285–294. doi: 10.1111/j.1574-695X.2009.00635.x

Fig. 4.

Fig. 4

C-di-GMP binds to PlzA of Borrelia burgdorferi with high specificity. R-PlzA, r-PlzA mutants R150D,R154D and R150K,R154K, and r-Rrp1 were expressed as N-terminal MBP fusions and were assessed for their ability to bind to [32P]c-di-GMP. The r-proteins used in the analyses are designated across the top of the panels. The ratio of competing nucleotide to [32P]c-di-GMP is indicated to the right (no competitor was added in the top panel). The second panel from the bottom was screened with [α-32P]GTP alone (with no c-di-GMP added) and the bottom panel is a Western blot using anti-MBP antibody.