Deregulation of gene expression by NUP98-DDX10. (a) For the 6 h time point, human CD34+ cells were nucleofected with either control pTracer vector, vector expressing NUP98-DDX10 or vector expressing NUP98-HOXA9. For the 3-day and 8-day time points, human CD34+ cells were retrovirally transduced with either control MSCV-IRES-GFP vector, vector expressing NUP98-DDX10, or vector expressing NUP98-HOXA9. Cells were sorted for GFP positivity and total RNA was subjected to microarray analysis. The experiment was performed two independent times and only genes that showed up- or down-regulation compared to control in both experiments were considered deregulated. Venn diagrams show the intersections of genes deregulated by NUP98-HOXA9 and NUP98-DDX10. (b) K562 cells were transfected by electroporation with a pGL4.11 luciferase construct driven by the KBTBD10 promoter or PLN promoter and either empty pTracer/CMV-Bsd vector (Control) or vector expressing NUP98-DDX10. Firefly luciferase activity was measured 48 h after transfection and normalized to a Renilla luciferase internal control and to empty pGL4.11 vector control. Error bars represent standard deviation.