Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Leukemia. 2010 Mar 25;24(5):1001–1011. doi: 10.1038/leu.2010.42

Figure 5.

Figure 5

Mutation of the YIHRAGTAR motif inhibits the ability of NUP98-DDX10 to induce transcription and cell proliferation. (a) Schematic representation of the NUP98-DDX10/3Q mutant produced by replacing the three arginine residues in the YIHRAGTAR motif with glutamines. (b) Immunoblotting with anti-HA antibody shows equivalent expression of NUP98-DDX10 and NUP98-DDX10/3Q in retrovirally transduced human CD34+ cells (upper panel). Anti-actin control is shown in the lower panel. (c) K562 cells were transfected by electroporation with a luciferase construct driven by the PLN promoter and either empty pTracer/CMV-Bsd vector (Control) or vector expressing NUP98-DDX10 or NUP98-DDX10/3Q. Firefly luciferase activity was measured 48 h after transfection and normalized to a Renilla luciferase internal control and to empty pGL4.11 vector control. The numbers represent averages of 3 independent experiments; error bars represent standard deviations. (d) Long-term liquid culture of primary human CD34+ cells transduced with NUP98-DDX10 or NUP98-DDX10/3Q. Cumulative cell numbers are expressed as percentages compared to unmutated NUP98-DDX10, which is taken as 100%. The numbers represent averages of 3 independent experiments; error bars represent standard deviation. The P value was obtained by comparing NUP98-DDX10 to NUP98-DDX10/3Q using a two-sample, equal variance, two-tailed distribution t-test.