Figure 7.

Binding of thin filament proteins to purified nebulin by far western blot analysis. Proteins (0.2 μg) adsorbed onto a nitrocellulose membrane were incubated in nebulin reaction solution (0.18 M KPO₄, 0.1 mM MgCl₂, 0.1 mM EGTA, and 0.5 M urea, pH 7.0) for 20 hours at 4°C. Amido black: amido black staining; +nebulin: incubation in nebulin (15 μg/ml), −nebulin: incubation in reaction solution alone.