Table 1.

Primers sequences and related amplicons for quantitative real-time RT-PCR or RT-PCR.

Genes	Primers (5′–3′)	Anneal temperature (°C)	Amplicon (bp)
β-actin	Forward: AAAGACCTGTACGCCAACAC	61	220
	Reverse: GTCATACTCCTGCTTGCTGAT
DNMT3A	Forward: TATTGATGAGCGCACAAGAGAGC	65	111
	Reverse: GGGTGTTCCAGGGTAACATTGAG
DNMT3B	Forward: GACTTGGTGATTGGCGGAA	64	271
	Reverse: GGCCCTGTGAGCAGCAGA
DNMT1	Forward: CCGAGTTGGTGATGGTGTGTAC	61	325
	Reverse: AGGTTGATGTCTGCGTGGTAGC
PTEN-q	Forward: CCGTTACCTGTGTGTGGTGATATC	60	100
	Reverse: AATGTATTTACCCAAAAGTGAAACATT