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Primers sequences for MSP and bisulfite sequencing analysis.
| Gene | Primers (5′–3′) | Anneal temperature (°C) |
|---|---|---|
| PTEN-Ma | Forward: TTTTTTTTCGGTTTTTCGAGGC | 59 |
| Reverse: CAATCGCGTCCCAACGCCG | ||
| PTEN-UMb | Forward: TTTTGAGGTGTTTGGGTTTTTGGT | 59 |
| Reverse: ACACAATCACATCCCAACACCA | ||
| PTEN-BGSc | Forward: ATTGGGTATGTTTAGTAGAGTT | 60 |
| Reverse: CAACTCTCAAACTTCCATCATA |
a: Methylated primer sequences used for MSP. b: Unmethylated primer sequences used for MSP. c: Primer sequences used for bisulfite genomic sequencing.
