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. 2010 May 14;86(5):805–812. doi: 10.1016/j.ajhg.2010.04.001

Figure 4.

Figure 4

Expression Analysis of the Short and Long BBS8 mRNA Isoforms

Expression levels of the short and long BBS8 mRNA isoforms (with or without exon 2a) in mouse tissue and retinal layers.

(A) The BBS8 mRNA without exon 2 was detected in all tissues tested, whereas exon 2a was only found in the retinas.

(B) Subsequent to laser capture microdissection and qRT-PCR, the BBS8 short message was detectable in all layers tested (GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; and RPE, retinal pigment epithelium), whereas the long isoform was found almost exclusively in the ONL. The y axis shows ratios of each transcript against GAPDH.