Table 1.
Validation of DNA Microarray Results by qPCR
Gene Name | Gene Symbol | Category | Microarray | qPCR |
---|---|---|---|---|
Paired-like homeodomain transcription factor 2 | Pitx2 | Transcriptional regulation | 9.33 | 7.15 ± 1.37 |
Homeo box A10 | Hoxa10 | Transcriptional regulation | −29.18 | −23.28 ± 3.55 |
Homeo box C10 | Hoxc10 | Transcriptional regulation | −69.66 | −11.13 ± 1.70 |
Homeo box B3 | Hoxb3 | Transcriptional regulation | −12.60 | −90.74 ± 14.83 |
CD59a antigen | Cd59a | Immune response | 10.89 | 79.62 ± 4.72 |
Interleukin 6 | Il6 | Immune response | 36.20 | 11.30 ± 2.65 |
Chemokine (C-X-C motif) ligand 14 | Cxcl14 | Immune response | 40.01 | 13.60 ± 1.46 |
CD109 antigen | Cd109 | Immune response | 8.64 | 2.76 ± 0.76 |
Laminin, alpha 2 | Lama2 | Extracellular | 17.96 | 25.23 ± 3.71 |
Plelotrophin | Ptn | Extracellular | −55.29 | −786.89 ± 85.16 |
Fibulin 1 | Fbln1 | Extracellular | −13.40 | −9.72 ± 5.62 |
Netrin 4 | Ntn4 | Extracellular | 20.95 | 17.92 ± 1.66 |
Connective tissue growth factor | Ctgf | Extracellular | 9.93 | 25.76 ± 1.66 |
Positive values indicate that levels are elevated in the EOM-Fibro compared with the LM-Fibro.
All qPCR values are significant at P < 0.01.