Fig. 3.

HAEC at passage 9 were stained for thrombomodulin (A) and smooth muscle cell actin (B) (immunofluorescence; original magnification ×200). In A, thrombomodulin stained in red is present in the cell membrane and, to a lesser extent, in the cytoplasm; nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) in blue. In B, main photograph depicts the absence of smooth muscle cell actin with positive DAPI staining for nuclei; inset shows smooth muscle cells, cytoplasm of which is positive for actin stained in green.