FIG. 1.

Sequence alignments of (A) the N-terminal region of eCry3.1Ab and variant engineered hybrid insecticidal proteins and (B) the C-terminal region of variant engineered hybrid insecticidal proteins. Solid underlining indicates “cap sequence” residues, gray shading indicates the engineered cathepsin G site, boxes indicate the conserved sequence blocks indicated beneath the boxes, the dotted vertical line indicates the junction between domain II (D II) and domain III (D III), and dashed underlining indicates the C-terminal Cry1Ab 38 amino acid “tail sequence.” Conserved blocks were defined as described by Höfte and Whiteley (11). Note that subscript designations of engineered hybrid insecticidal proteins are used to distinguish different proteins in the present work and do not correspond to any other sequence numbering convention.