TABLE 2.
Genus (class or phylum) and isolatea | Isolation conditions |
Denitrificationc |
Aerobic growthd | SSU rRNA (rrs) | Primer set (GenBank accession no.)e |
GenBank accession no. for draft genome sequence |
||||
---|---|---|---|---|---|---|---|---|---|---|
Electron donorb | Nitrate concn (mM) | N2O production | N2 production | nirK | nosZ | nirK | nosZ | |||
Afipia (Alphaproteobacteria) | ||||||||||
1NLS2* | Lactate | 0.5 | — | — | — | FJ851425 | 1 | 6 | ||
2LS1 | Lactate | 1 | — | — | — | FJ851426 | 2 | 6 | ||
3AS3* | Acetate | 10 | Neg | Pos | Pos | FJ851427 | 1 (GQ404513) | 6 (GQ404519) | ||
4AS1 | Acetate | 25 | Neg | Pos | — | FJ851428 | 1 (GQ404514) | 6 | ||
4LS2 | Lactate | 25 | Neg | Pos | — | FJ851429 | 1 | 6 | ||
4LS4 | Lactate | 25 | — | — | — | FJ851430 | 2 | 6 (GQ404520) | ||
4LS5 | Lactate | 25 | Neg | Pos | — | FJ851431 | 1 | 6 | ||
Hyphomicrobium (Alphaproteobacteria) | ||||||||||
1NES1* | Ethanol | 0.5 | Neg | Pos | VW | FJ851438 | 4 (GU814013) | 6 (GQ404521) | ||
2NES1* | Ethanol | 1 | Neg | Pos | — | FJ851439 | 4 | 6 (GQ404522) | ||
Rhodanobacter (Gammaproteobacteria) | ||||||||||
2AS1* | Acetate | 1 | Pos | Pos | — | FJ851443 | 3 (GQ404515) | 7 (GQ404523) | ||
2APBS1* | APB | 1 | — | — | Pos | FJ851444 | 3 (GQ404516) | 7 (GQ404524) | GU233006, GU233007 | GU233008 |
3LS1 | Lactate | 10 | Pos | Pos | — | FJ851445 | 3 | 7 | ||
Intrasporangium (Actinobacteria) | ||||||||||
3APBS1* | APB | 10 | Pos | Neg | Pos | FJ851441 | Neg | Neg | ||
3AS1 | Acetate | 10 | Pos | Neg | — | FJ851440 | Neg | Neg | ||
4LS1* | Lactate | 25 | Pos | Neg | — | FJ851442 | Neg | Neg | GU233009 | |
Bacillus (Firmicutes) | ||||||||||
1NLA3E* | Lactate | 0.5 | Pos | Neg | Pos | FJ851432 | Neg | Neg | ||
2NLA3E* | Lactate | 1 | — | — | — | FJ851433 | Neg | Neg | ||
AS31 | Lactate | 0.5 | — | — | — | FJ851434 | Neg | Neg | ||
A3S2 | Lactate | 0.5 | — | — | — | FJ851435 | Neg | Neg | ||
A3S3 | Lactate | 0.5 | — | — | — | FJ851436 | Neg | Neg | ||
AS34 | Lactate | 0.5 | — | — | — | FJ851437 | Neg | Neg |
An asterisk indicates that the isolate is presented in the phylogenetic tree (Fig. 1).
Electron donor concentrations: acetate, 10 mM; lactate, 10 mM; ethanol, 20 mM; acetate, propionate, butyrate (APB), 10 mM.
Production of nitrous oxide or nitrogen gas was tested by gas chromatography, as described in Materials and Methods. Neg, negative; Pos, positive; —, not tested.
Pos, positive growth on agar medium under aerobic conditions; VW, very weak growth; —, not tested.