TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid and function | Relevant genotype or descriptiona | Reference or source |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli | ||
| DH5α-λpir | φ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1/λpir | 48 |
| WM3064 | thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)] | W. Metcalf, University of Illinois, Urbana-Champaign |
| BTH101a | F−cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA mcrB1 | Euromedex, France |
| Shewanella oneidensis MR-1 | ||
| S79 | Wild type | 72 |
| S143 | ΔSO_0577 (ΔarcS) | This study |
| S838 | Wild type (“knock-in” complementation of ΔarcS) | This study |
| S313 | ΔSO_1327 (ΔhptA) | This study |
| S802 | Wild type (knock-in complementation of ΔhptA) | This study |
| S318 | ΔSO_3988 (ΔarcA) | This study |
| S805 | Wild type (knock-in complementation of ΔarcA) | This study |
| S315 | Δarc ΔhptA | This study |
| S320 | ΔarcS ΔarcA | This study |
| S836 | ΔhptA ΔarcA | This study |
| S834 | ΔarcS ΔhptA ΔarcA | This study |
| Plasmids | ||
| pNPTS138 | mobRP4+sacB ColE1 ori Kmr | M. R. Alley, unpublished data |
| pUC18R6KT-mini-Tn7T | mobRP4+ori-R6K TnR/TnL Apr | 13 |
| pNPTS138-R6KT | mobRP4+ori-R6K sacB; suicide plasmid for in-frame deletions; Kmr | This study |
| Construction of in-frame deletions and complementation in Shewanella sp. | ||
| pGP704Sac28Km | mobRP4+ori-R6K sacB; suicide plasmid for in-frame deletions; Kmr | Chengyen Wu, unpublished data |
| pGP704Sac28Km-ΔSO_0577 | SO_0577 (arcS) deletion fragment in pGP704Sac28Km | This study |
| pGP704Sac28Km-KI-SO_0577 | SO_0577 (arcS) complementation fragment in pGP704Sac28Km | This study |
| pNPTS138-R6KT-ΔhptA | hptA deletion fragment in pNPTS138R6KT | This study |
| pNPTS138-R6KT-KI-hptA | hptA complementation fragment in pNPTS138R6KT | This study |
| pNPTS138-R6KT-ΔarcA | arcA deletion fragment in pNPTS138R6KT | This study |
| pNPTS138-R6KT-KI-arcA | arcA complementation fragment in pNPTS138R6KT | This study |
| pNPTS138-R6KT-ΔSputCN32_3300 | SputCN32_3300 (arcS) deletion fragment in pNPTS138R6KT | This study |
| pNPTS138-R6KT-KI-SputCN32_3300 | SputCN32_3300 complementation fragment in pNPTS138R6KT | This study |
| pBAD33 | ori-p15a araC PBAD Cmr | 23 |
| pBAD33-RBS-SO0577 | SO_0577 (arcS) in pBAD33 | This study |
| pBAD33-RBS-hptA | hptA in pBAD33 | This study |
| pBAD33-RBS-arcA | arcA in pBAD33 | This study |
| Overproduction of S. oneidensis MR-1 Arc components | ||
| pBAD-HisA | Invitrogen | |
| pBAD-HisA-SO_0577-646 | SO_0577 C-terminal coding region (aa 646-1188) in pBADHisA | This study |
| pBAD-HisA-arcA | arcA in pBADHisA | This study |
| pBAD-HisA-arcA-D54N | arcA(D54N) in pBADHisA | This study |
| pBAD-HisA-SO_3457-181 | SO_3457 C-terminal coding region (aa 181-929) in pBADHisA | This study |
| pGEX4T-1 | ori-pBR322 Plac Apr | |
| pGEX4T-1-hptA | hptA in pGEX4T-1 | This study |
| pGEX4T-1-hptA-H62A | hptA(H62A) in pGEX4T-1 | This study |
| Bacterial two-hybrid constructs | ||
| pUT18 | ori-ColE1 Plac T18 for N-terminal fusion; Apr | Euromedex, France |
| pUT18C | ori-ColE1 PlacT18 for C-terminal fusion; Apr | Euromedex, France |
| pKT25 | ori-p15a Plac T25 for C-terminal fusion; Kmr | Euromedex, France |
| pKNT25 | ori-p15a Plac T25 for N-terminal fusion; Kmr | Euromedex, France |
| pUT18-SO_0577-364 | SO_0577 (arcS) C-terminal region (aa 364-1188) in pUT18 | This study |
| pUT18C-SO_0577-364 | SO_0577 (arcS) C-terminal region (aa 364-1188) in pUT18C | This study |
| pKT25-SO_0577-364 | SO_0577 (arcS) C-terminal region (aa 364-1188) in pKT25 | This study |
| pUT18-hptA | hptA in pUT18 | This study |
| pUT18C-hptA | hptA in pUT18C | This study |
| pKT25-hptA | hptA in pKT25 | This study |
| pKNT25-hptA | hptA in pKNT25 | This study |
| pUT18C-arctA | arcA in pUT18C | This study |
| pKT25-arcA | arcA in pKT25 | This study |
| pKNT25-arcA | arcA in pKNT25 | This study |
| pUT18C-SO_3457-172 | SO_3457 (barA) C-terminal region (aa 172-929) into pUT18C | This study |
| pKT25-SO_3457-172 | SO_3457 (barA) C-terminal region (aa 172-929) into pKT25 | This study |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.