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(A) Tricine-SDS-PAGE of purified recombinant EntL50A and EntL50B after silver staining. (B) Western blotting using rabbit polyclonal antibodies with specificity for EntL50A (anti-LR1-KLH) and EntL50B (anti-LR2-KLH). (C) Antimicrobial activity after gel overlay with the indicator strain Pc. damnosus CECT4797. Lane 1, purified EntL50A; lane 2, purified EntL50B. SeeBlue prestained standard molecular mass marker (Invitrogen) band sizes (M) are indicated on the left.
