TABLE 2.
Plasmidb | Selection marker | Size (kb) | Log10 no. of transformants/μg plasmid DNAc |
|
---|---|---|---|---|
NCTC9343 | YCH46 | |||
pLYL05 | Cfxr | 7.55 | 4.28 ± 0.13 | 4.01 ± 0.59 |
pNLY1 | Cmr | 7.76 | 3.34 ± 0.61 | 1.22 ± 0.53 |
pVAL-1 | Emr | 11 | 0.05 ± 0.09 | 1.66 ± 0.37 |
pVAL-2 | Cfxr | 10.1 | 1.27 ± 1.10 | 2.83 ± 0.15 |
B. fragilis cells at mid-exponential growth phase were electroporated with 1.0 μg DNA of each plasmid indicated. After 12 h of postpulse incubation, cells were spread onto GAM agar plates containing the selective antibiotic corresponding to the plasmid used.
All plasmids were purified from E. coli laboratory strain HB101.
The data are means ± standard deviations.