TABLE 3.
Electrotransformation of Bacteroides species using in vivo methylation
| Straina | Plasmidb | Log 10 no. of transformants/μg plasmid DNAc |
|
|---|---|---|---|
| Without in vivo methylation | With in vivo methylation | ||
| Bacteroides fragilis NCTC9343 | pLYL05 | 5.36 ± 0.04 | 7.23 ± 0.05 |
| Bacteroides fragilis YCH46 | pLYL05 | 4.42 ± 0.34 | 6.25 ± 0.08 |
| Bacteroides thetaiotaomicron VPI-5482 | pNLY1 | 3.43 ± 0.65 | 4.45 ± 0.25 |
| Bacteroides vulgatus ATCC 8482 | pLYL05 | 3.15 ± 0.16 | 2.56 ± 0.11 |
a
Competent cells were prepared from 48-h cultures and electroporated with 0.45 μg DNA of each plasmid indicated. After 3 h of postpulse incubation, cells were spread onto GAM agar plates containing selective antibiotics corresponding to the plasmid used.
b
All plasmids were purified from E. coli laboratory strain HB101. pNLY1 was used for B. thetaiotaomicron strain VPI-5482 as this strain is cefoxitin resistant.
c
The data are means ± standard deviations calculated from three independent experiments.