FIG. 1.

Schematic diagrams of the wild-type enzymes, chimeric enzymes, and chimeric scaffoldins that were used in this study. The modular notation, structure, and molecular mass of each protein are indicated. White indicates a C. thermocellum cellulosome-derived component; black indicates a C. thermocellum noncellulosomal component; and gray indicates a B. cellulosolvens cellulosome-derived component. In the modular notation of the enzymes, the numbers indicate the family of the catalytic domain; R, I, S, and Y indicate the original names of the enzymes (Cel9R, Cel9I, Cel48S, and Cel48Y, respectively); and b and t indicate the source of the dockerin module (B. cellulosolvens from ScaB and C. thermocellum from Cel48S, respectively). B and T indicate the source of the divergent cohesins (B, the first cohesin from ScaA of B. cellulosolvens; T, the third cohesin from CipA of C. thermocellum). An asterisk indicates a converted enzyme (an enzyme converted either from cellulosomal to noncellulosomal, in which the native dockerin was replaced by a cellulose-binding CBM, or vice versa).