FIG. 2.

Comparative degradation of Avicel by individual family 48 and family 9 enzymes. Cellulosomal enzymes were converted to the free mode, and free enzymes were converted to the cellulosomal mode. The compositions of the reaction mixtures in this figure and Fig. 3 and 4 are indicated by diagrams above the bars. A white structure indicates a cellulosome-derived enzyme, and a black structure indicates a non-cellulosome-derived enzyme. Open bars, 48S and 9R catalytic modules in either “WT-cellulosomal,” “mock-free” or “converted-free” mode; filled bars, 48Y-CBM and 9I-CBM catalytic modules in either the “WT-free,” “converted-cellulosomal,” or “mock-free” mode. For definitions of enzyme systems see Table 2. The results of two independent experiments are shown. Triplicate reactions were carried out, and standard deviations are indicated by error bars. The relative activity was determined by comparison with the activity of the WT-free enzyme 9I-CBM, as described in Materials and Methods.