FIG. 3.

Comparative degradation of Avicel by combinations of family 48 and family 9 enzymes. Open bars, conversion from cellulosomal mode to free mode (the cellulosomal enzyme 48S and 9R catalytic modules in “WT-cellulosomal,” “mock-free,” and “designer-WT” modes were converted into the “converted-free” mode); filled bars, conversion from free mode to cellulosomal mode (the free 48Y and 9I catalytic modules in a “WT-free” mode were converted into a cellulosomal mode, either “converted-cellulosomal,” “mock-free,” or “designer-converted”). For definitions of enzyme systems see Table 2. The results of two independent experiments are shown. Triplicate reactions were carried out, and standard deviations are indicated by error bars. The relative activity was determined by comparison with the activity of the WT-free enzymes 48Y-CBM and 9I-CBM, as described in Materials and Methods.