TABLE 1.
Expression conditions, solubilities, and yields of the recombinant proteins used in this study
| Plasmid | Recombinant protein | Expression conditions |
Solubility | Final yield (mg protein/ liter culture) | ||||
|---|---|---|---|---|---|---|---|---|
| Temp (°C) | Time (h) | IPTG concn (mM) | Calcium (2 mM) | Length of heating (min)a | ||||
| pET21a | Cel9I (9I-CBM) | 37 | 3 | 0.2 | − | 30 | High | 24 |
| pET21a | Cel48Y (48Y-CBM) | 16 | 18 | 1 | − | 30 | High | 40 |
| pET21a | *9I-b | 37 | 3 | 0.2 | + | 30 | High | 20 |
| pET21a | *48Y-t | 37 | 3 | 0.2 | + | 30 | High | 10 |
| pET28a | Cel48S (48S-t) | 37 | 3 | 0.2 | + | 20 | Low | 0.5 |
| pET28a | 9R-b | 37 | 3 | 0.2 | + | 15 | High | 4 |
| pET21a | *48S-CBM | 20 | 18 | 0 | − | 30 | Low | 2.3 |
| pET21a | *9R-CBM | 37 | 3 | 0.2 | − | 30 | High | 17 |
a
Most proteins were heated at 60°C; the only exception was Cel48S, which was heated at 50°C.