TABLE 1.
Biophysical properties of OAKs and polylysines and their bacterial capture capacities
| Sequence | Designation | No. of residues | Qa | Hb | MIC (μM) for speciesc: |
Bound E. coli (CFU/mg resin)d | |||
|---|---|---|---|---|---|---|---|---|---|
| E. coli | P. aeruginosa | S. aureus | B. cereus | ||||||
| LK-cKcKcKcKcKcKcKa | C12K-7α8 | 16 | 8 | 47.5 | 3.1 | 6.2 | 50 | 12.5 | (1 ± 0.5) × 103 |
| KKKKKKKKa | Poly-K8 | 8 | 9 | 20 | >50 | >50 | >50 | >50 | (9 ± 1) × 102 |
| KKKKKKKKKKKKKKKa | Poly-K15 | 15 | 16 | 22 | >50 | >50 | >50 | >50 | (1 ± 0.6) × 103 |
| K-cKcKcKcKcKcKcKa | K-7α8 | 15 | 9 | 34 | >50 | >50 | >50 | >50 | (2 ± 0.6) × 102 |
| K-lKlKlKlKlKlKlKa | K-7α12 | 15 | 9 | 50 | >50 | >50 | >50 | >50 | (1 ± 0.3) × 106 |
a
Molecular charge at physiological pH.
b
Estimated hydrophobicity (percent acetonitrile eluent) as determined by reverse-phase HPLC.
c
Minimal concentration that induced 100% inhibition of proliferation after 24 h of incubation. Note that MICs of C12K-7α8 are from reference 27. Values represent the means from two independent experiments performed in duplicate (the absence of the standard deviation reflects consistency).
d
Bacterial binding assessed after 30 min of incubation as described in Materials and Methods. L, lauryl; K, lysyl; c, aminocaprilyl; l, aminolauryl; a, amide.