TABLE 1.
Sensitivity of sandwich ELISAs for the detection of BoNT/A, BoNT/B, and the corresponding complexes in buffer and raw milk
| Toxin | LODa (pg ml−1) in: |
|
|---|---|---|
| Bufferb | Raw milk | |
| BoNT/A | 53 ± 12 | 47 ± 9 |
| BoNT/A complex | 650 ± 221 | 558 ± 222 |
| BoNT/B | 102 ± 26 | 136 ± 51 |
| BoNT/B complex | 653 ± 184 | 698 ± 245 |
a
Limit of detection (LOD) was calculated on the basis of the arithmetic mean and the 3-fold standard deviation of the results for 12 to 16 blank samples for three interassay standard curves.
b
PBS containing 0.1% bovine serum albumin.