. 2010 Apr 2;76(10):3293–3300. doi: 10.1128/AEM.02937-09

TABLE 2.

Toxic activities of BoNT/A, BoNT/B, and the corresponding BoNT complexes in milk after pasteurization

Toxin	Time sample held at 72°C (s)	Dilution of BoNT-spiked milk	No. of mice surviving/no. of mice tested	MLD before heating/ MLD after heating	Reduction in toxic activity (%)
BoNT/A
	Not heated	—^a	0/2	10,000/10,000	0
	1	—	2/2	10,000/<1	>99.99
	5	—	2/2	10,000/<1	>99.99
	15	—	2/2	10,000/<1	>99.99
	30	—	2/2	10,000/<1	>99.99
BoNT/A complex
	Not heated	—	0/2	10,000/10,000	0
	1	—	0/2
		1:10	0/5
		1:50	5/5	200/<1	>99.5
		1:100	2/2
	15	—	0/2
		1:10	0/5
		1:50	5/5	200/<1	>99.5
	180	—	0/5
		1:10	5/5	1,000/<1	>99.9
BoNT/B
	Not heated	—	0/2	10,000/10,000	0
	1	—	2/2	10,000/<1	>99.99
	5	—	2/2	10,000/<1	>99.99
	15	—	2/2	10,000/<1	>99.99
	30	—	2/2	10,000/<1	>99.99
BoNT/B complex
	Not heated	—	0/2	10,000/10,000	0
	1	—	0/2
		1:10	0/2
		1:50	0/5
		1:100	5/5	100/<1	>99.0
	15	—	0/2
		1:10	0/5
		1:50	5/5	200/<1	>99.5
	180	—	0/5
		1:10	5/5	1,000/<1	>99.9

—, no dilution.