FIG. 2.

Fluorimetric measurements. (a) Intensities of SYTO 9 (live cells) and PI (dead cells) emission along the S. coelicolor developmental cycle. The cellular concentration (mg of protein per ml) is also shown. (b) Variation of the SYTO 9/PI ratio along the developmental cycle. Antibiotic (actinorhodin and undecylprodigiosin) production is indicated. MI, first compartmentalized mycelium. MII, second multinucleated mycelium. Arrows indicate maximum SYTO 9 intensities (live cells) corresponding to MI (15 h) and MII (100 h) mycelia. Data are presented as averages and standard deviations from two biological replicates measured three times each (three methodological replicates). R.U., relative units. See also Fig. S2 in the supplemental material.