TABLE 2.
Plasmids used in this study
| Plasmid | Relevant propertiesa | Source |
|---|---|---|
| pACYC184 | Tcr Cmr, p15A ori, 4.2 kb | New England Biolabs |
| pCar184 | Tcr, Erwinia uredovora crt operon (crtEXYIB) under gntT104 promoter cloned between EcoRI of site of pACYC184, 11.1 kb | This study |
| pLyc184 | Tcr, deletion of crtXY genes from pCar184, 8.7 kb | This study |
| pTrc99A | Apr, trc promoter, ColE1 ori, 4.1 kb | Amersham Pharmacia |
| pTrcDx | Apr, trc promoter, dxs gene cloned at KpnI and EcoRI sites of pTrc99A, 4.7 kb | This study |
| pTrcDx-idi | Apr, trc promoter, idi gene cloned at KpnI and XbaI sites of pTrcDx under its own RBS, 6.6 kb | This study |
| pTrcDx-fbaA | Apr, trc promoter, fbaA gene cloned at KpnI and XbaI sites of pTrcDx under its own RBS, 7.1 kb | This study |
| pTrcDx-tpiA | Apr, trc promoter, tpiA gene cloned at KpnI and XbaI sites of pTrcDx under its own RBS, 6.8 kb | This study |
| pTrcDx-pfkA | Apr, trc promoter, pfkA gene cloned at XbaI and PstI sites of pTrcDx under its own RBS, 7.1 kb | This study |
| pTrcDx-pgi | Apr, trc promoter, pgi gene cloned at XbaI and PstI sites of pTrcDx under its own RBS, 7.7 kb | This study |
| pTrcDx-icdA | Apr, trc promoter, icdA gene cloned at XbaI site of pTrcDx under its own RBS, 7.3 kb | This study |
| pTrcDx-mdh | Apr, trc promoter, mdh gene cloned at XbaI site of pTrcDx under its own RBS, 7.0 kb | This study |
| pTrcDx-idi-fbaA | Apr, trc promoter, fbaA gene cloned at XbaI site of pTrcDx-idi under its own RBS, 7.7 kb | This study |
| pTrcDx-idi-tpiA | Apr, trc promoter, tpiA gene cloned at XbaI site of pTrcDx-idi under its own RBS, 7.4 kb | This study |
| pTrcDx-idi-mdh | Apr, trc promoter, mdh gene cloned at XbaI site of pTrcDx-idi under its own RBS, 7.9 kb | This study |
a
RBS, ribosome binding site.