TABLE 3.
List of key genes and enzymes investigated based on the FSEOF simulation
| Gene | Enzyme | Functional category |
|---|---|---|
| acnAB | Aconitase | TCA cycle |
| gltA | Citrate (CIT) synthase | |
| fumAB | Fumarase | |
| icdAa | Isocitrate (ICIT) dehydrogenase (NADP) | |
| mdha,c | Malate (MAL) dehydrogenase | |
| sdhABCD | Succinate (SUC) dehydrogenase | |
| sucCD | Succinyl-CoA (SUCOAS) synthetase (ADP-forming) | |
| sucAB | 2-Oxoglutarate (AKG) dehydrogenase | |
| sdhABCD | Succinate dehydrogenase | |
| dxr | 1-Deoxy-d-xylulose 5-phosphate reductoisomerase | Methylerythritol phosphate pathwayb |
| dxsc | 1-Deoxyxylulose-5-phosphate synthase | |
| idia | Isopentenyl diphosphate (IPDP) isomerase | |
| ispAa | Geranyltranstransferase/dimethylallyltranstransferase | |
| ispD | 4-Diphosphocytidyl-2C-methyl-d-erythritol synthase | |
| ispE | 4-Diphosphocytidyl-2-C-methylerythritol kinase | |
| ispF | 2C-Methyl-d-erythritol 2,4-cyclodiphosphate synthase | |
| ispG | 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | |
| ispH | 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase | |
| crtE | Geranylgeranyl pyrophosphate (GGPP) synthase | Heterogeneous genes for lycopene synthesis |
| crtB | Phytoene (PHYTO) synthetase | |
| crtI | Phytoene dehydrogenase | |
| fbaAa | Fructose-bisphosphate (FBP) aldolase | Glycolysis |
| pfkABa | Phosphofructokinase | |
| pgia | Glucose-6-phosphate (G6P) isomerase | |
| tpiAa,c | Triose-phosphate isomerase |
a
A gene that was overexpressed in this study.
b
Enhancing fluxes of the methylerythritol phosphate pathway has a positive effect on lycopene production. Among them, the products of dxs, idi, and ispA were previously shown to enhance lycopene production.
c
A key gene that was newly identified as an amplification target by using FSEOF.