TABLE 1.
PCR primers used in this study
| Process | Target gene/target enzyme | Primer | Sequence (5′-3′) | Reference |
|---|---|---|---|---|
| Ammonia oxidation (Bacteria) | amoA/ammonia monooxygenase | amoA-1F* | GGGGHTTYTACTGGTGGT | 53 |
| amoA-2R | CCCCTCKGSAAAGCCTTCTTC | |||
| Ammonia oxidation (Archaea) | amoA/ammonia monooxygenase | Arch-amoAF | STAATGGTCTGGCTTAGACG | 8 |
| Arch-amoAR | GCGGCCATCCATCTGTATGT | |||
| Nitrate reduction | narG/respiratory nitrate reductase | narG1960f | TAYGTSGGCARGARAA | 60 |
| narG2659r | TTYTCRTACCABGTBGC | |||
| Methane oxidation | mxaF/methanol dehydrogenase | mxa1003f | GCGGCACCAACTGGGGCTGGT | 27 |
| mxa1561r | GGGCAGCATGAAGGGCTCCC | |||
| Methanogenesis | mcrA/methyl-coenzyme M reductase | MLf | GGTGGTGTMGGATTCACACARTAYGCWACAGC | 39 |
| MLr | TTCATTGCRTAGTTWGGRTAGTT | |||
| mcrA/methyl-coenzyme M reductase | ME1f | CGMATGCARATHGGWATGTC | 27 | |
| ME2r | TCATKGCRTAGTTDGGRTAGT | |||
| Anaerobic degradation of | bssA/benzylsuccinate synthase | 7772f | GACATGACCGACGCSATYCT | 77 |
| alkyl aromatics | 8546r | TCGTCGTCRTTGCCCCAYTT | ||
| Dissimilatory nitrate | nrfA/cytochrome c nitrite reductase | nrfAF1 | GCNTGYTGGWSNTGYAA | 60 |
| reduction to ammonia | nrfA7R1 | TWNGGCATRTGRCARTC |