TABLE 3.
Closest relatives in the GenBank database for partial narG (652 bp) and mxaF (554 bp) DNA sequences obtained from well 36a
| Targeted gene | Sequence designation | Best BLAST match or closest match in cultured organism | Maximum % identity | Accession no. |
|---|---|---|---|---|
| narG | JMYnarG02 | Uncultured bacterium clone RT-250_16 | 84 | DQ481115 |
| Geobacter metallireducens GS-15 | 82 | CP000148 | ||
| narG | JMYnarG03 | Uncultured bacterium partial narG | 88 | AM408519 |
| Geobacter metallireducens GS-16 | 84 | CP000148 | ||
| narG | JMYnarG05 | Uncultured bacterium clone GRAMO27 | 85 | AY955194 |
| Methylobacterium sp. strain 4-46 | 81 | CP000943 | ||
| mxaF | JMYmxaF01 | Methylocystis sp. strain 5FB2 | 98 | EF212330 |
a
Libraries were prepared from the August 2006 samples. For narG, a library of 9 clones led to three RFLP patterns and three gene sequences. For mxaF, a library of 8 clones led to a single RFLP pattern and a single gene sequence.