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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Regen Med. 2009 Nov;4(6):823–833. doi: 10.2217/rme.09.51

Figure 1. Cardiac stem cells from transgenic mice express αMHC driven transgenes in vivo and in culture.

Figure 1

(A) Immunolabeling of adult MHCGFP mouse heart section shows colocalization of c-kit (red) and GFP (green) staining. Desmin (blue) also colocalizes with GFP in mature myocytes. Scale bar is 10 μM. (B) CSCs isolated from MHCGFP, MHCAkt-nuc and MHCPim-wt were cultured in growth media and harvested for RNA isolation and cDNA synthesis. cDNA was amplified using primers specific for the transgene inserted into each CSC line. (C) Flow cytometric analysis of NTG, MHCPim-wt and MHCAkt-nuc CSCs either untreated (left) or hydrogen peroxide treated (right) stained for annexin V and 7-AAD apoptosis markers (representative of n = 2 independent experiments). (d) Fold-increase quantitation of annexin V+/7-AAD+ CSCs after hydrogen peroxide treatment in (C). (e) Trypan blue exclusion hemocytometer counts of NTG, MHCPim-wt and MHCAkt-nuc CSCs seeded at equal amounts on day 0 and counted 2 or 4 days later (representative of n = 3 independent experiments).

7-AAD: 7-amino-actinomycin D; CSC: Cardiac stem cell; GFP: Enhanced green fluorescent protein; MHC: Myosin heavy chain; NTG: Nontransgenic.