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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Regen Med. 2009 Nov;4(6):823–833. doi: 10.2217/rme.09.51

Figure 2. Nontransgenic cardiac stem cells express tagged fluorescent marker proteins upon transfection with plasmid constructs under the control of the αMHC promoter.

Figure 2

Nontransgenic cardiac stem cells were cultured in growth media, transfected with plasmid constructs, allowed to express for 48 h and fixed. (A) CMV-Td-Tomato-Flag construct (left). Cells were transfected with a Tomato-Flag construct under the viral CMV promoter as a control and stained with c-kit or Flag-tag (green) and Topro (blue), td-Tomato protein fluorescence (red) (right). (B) αMHC-YFP-HA construct (left). Cells were transfected with a YFP-HA construct under the αMHC promoter and stained with c-kit or HA (red), Topro (blue) and YFP fluorescence (green) (right). (C) Diagram of αMHC-GFP construct (left). Cells were transfected with a GFP construct under control of the αMHC promoter and stained with c-kit (red), Topro (blue) and GFP protein fluorescence (green) (right). Scale bar is 40 μm.

CMV: Cytomegalovirus; eGFP: Enhanced Green fluorescent protein; MHC: Myosin heavy chain; YFP: Yellow fluorescent protein.