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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Regen Med. 2009 Nov;4(6):823–833. doi: 10.2217/rme.09.51

Figure 3. Cardiac stem cells express transgenes controlled by the αMHC promoter in the appropriate subcellular localization in culture by confocal microscopy.

Figure 3

(A) NTG and (B) MHCPim-wt CSCs cultured in growth media, fixed and immunolabeled with antibodies to Pim (green), c-kit (red) and Topro (blue). MHCPim-wt CSCs show increased Pim expression localized to the cytoplasm. (C) NTG and (d) MHCAkt-nuc CSCs cultured in growth media, fixed and immunolabeled with Myc-tag (green), c-kit (red) and Topro (blue). MHCAkt-nuc CSCs show nuclear-localized myc-tag expression. Scale bar is 40 μm.

CSC: Cardiac stem cell; MHC: Myosin heavy chain; NTG: Nontransgenic.