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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Regen Med. 2009 Nov;4(6):823–833. doi: 10.2217/rme.09.51

Figure 4. Cardiomyogenic differentiation allows for persistent αMHC driven transgene expression.

Figure 4

(A) MHCPim-wt and (B) NTG CSCs differentiated on fixed NRCMs for 7 days, fixed and immunolabeled for Pim (green), α-sarcomeric actinin (red) and Topro (blue). MHCPim-wt differentiated CSCs express more Pim protein. (C) MHCPim-wt and (D) NTG CSCs differentiated on fixed NRCMs for 7 days, fixed and immunolabeled for GFP (green), α-sarcomeric actinin (red) and Topro (blue). MHCPim-wt differentiated CSCs express GFP. (E) MHCAkt-nuc and (F) NTG CSCs differentiated on fixed NRCMs for 7 days, fixed and immunolabeled for myc-tag (green), α-sarcomeric actinin (red) and Topro (blue). MHCAkt-nuc differentiated CSCs express increased nuclear-localized myc-tag. Scale bar is 40 μm. (G) RT-qPCR analysis of NTG CSCs postdifferentiation on live NRCMs shows an increase in relative mRNA levels of troponin T (left), tropomyosin (middle) and α-smooth muscle actin (right) compared with undifferentiated CSCs.

CSC: Cardiac stem cell; GFP: Green fluorescent protein; MHC: Myosin heavy chain; NRCM: Neonatal rat cardiomyocyte; NTG: Nontransgenic.