Table 2.
Antibodies
| Antibody/ Antigen |
Host Species, Dilution |
Source | Specificity* |
|---|---|---|---|
| Hic-5/ARA55 | Rabbit 1:300 | GenWay SanDiego, CA |
Hic-5 cell adhesion redox sensitive |
| Paxillin | Rabbit 1:300 | Epitomics Burlingame, CA |
Paxillin, cell adhesion |
| pY31 Paxillin | Rabbit 1:300 | Epitomics | Modification during FA formation |
| pY116 Paxillin | Rabbit 1:100 | Biosource Camarillo, CA |
Modification during FA formation by Crk/Src and FAK after integrin activation |
| pS126 Paxillin | Rabbit 1:100 | Biosource | Modified after Raf stimulation in GSK3 pathway |
| pS178 Paxillin | Rabbit 1:100 | Biosource | Modified by cdc2 kinase during mitosis, JNK during cell migration |
| β-Amyloid | Mouse 1:300 | Dako Carpinteria, CA |
Plaques |
| AT8 | Mouse 1:300 | Pierce Rockford, IL |
Hyperphosphorylated tau |
| GFAP | Mouse 1:1000 | Dako | Activated astrocytes |
| CD68 | Mouse 1:100 | Dako | Mature and immature Microglia |
| TO-PRO-3 | 1:300 | Invitrogen Carlsbad, CA |
Nuclei |
*
As reported on the company data information sheet. FA = focal adhesion.