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. 2010 Mar 10;151(5):2162–2170. doi: 10.1210/en.2009-1159

Figure 1.

Figure 1

Exogenous bacterial PLD primes glomerulosa cells to respond to a subsequent exposure to AngII with enhanced secretion. Primary cultures of bovine adrenal glomerulosa cells in serum-free medium for 20–24 h were incubated for a 30-min control period. Cells were then treated with 10 nm AngII (□) or 0.1 IU/ml PLD (○) as indicated, followed by a 40-min washout and exposure to 10 nm AngII as illustrated. Aldosterone secretion was assayed using a solid-phase RIA kit. Values represent the means ± sem of three independent experiments, expressed as the percent maximal response. ***, P < 0.001; **, P < 0.01; *, P < 0.05 vs. control (shown at time = 30 min); ††, P < 0.01; †††, P < 0.001 vs. the corresponding time point during the first AngII treatment. The maximal responses, determined in each experiment as the maximum aldosterone secretory rate and occurring in one experiment during the second AngII exposure and in two during the AngII exposure after PLD pretreatment, were 57.5, 115.9, and 27.3 pg/ml · min.