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The effect of conditioned medium (CM) from GH-treated macrophages on the differentiation of 3T3-L1 preadipocytes is restricted to the induction phase of adipogenesis. 3T3-L1 cells were incubated with CM (mixed 1:1 with culture medium) from either macrophages (CM) or GH-CM-Mac for the first 2 d (A) or last 6 d (B) after addition of the differentiation cocktail mixture. Differentiation of adipocytes was monitored by staining with Oil Red O staining 8 d after differentiation. C, Control.
