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. 2010 Mar 22;151(5):2140–2150. doi: 10.1210/en.2010-0160

Figure 1.

Figure 1

Inhibition or ablation of AR prevents HG- and/or TNF-α-induced VSMC proliferation. A, Growth-arrested VSMC were preincubated with 10 μm sorbinil or carrier for 24 h in the presence or absence of HG and/or TNF-α. B, VSMC were transfected with AR siRNA or scrambled AR siRNA for 24 h at 37 C in the presence or absence of HG. Subsequently, the cells were stimulated with TNF-α (2 nm) for another 24 h. Cell viability was determined by MTT assay. Inset in B, Western blot analysis of untransfected (c), scrambled (s), and AR siRNA-transfected (a) cell extracts for determination of AR protein levels. Bars, Mean of five individual experiments (n = 5) ± sem; #, P < 0.001 compared with treatment without the inhibitor or scrambled AR siRNA-transfected cells; *, P < 0.001 compared with HG- and/or TNF-α-treated cells. NG, Normal glucose.