Figure 2.

The lack of UCP2 increases total body fat in response to ghrelin treatment. We measured total body fat, muscle, and fluid using NMR spectroscopy and calculated the fold increase by comparing measurements before and after ghrelin treatment. A, Ghrelin significantly increased total body fat in both ucp2^+/+ and ucp2^−/− mice (n = 6); however, the increase in ucp2^−/− was further significantly elevated when compared with ucp2^+/+ mice. B and C, No differences were observed in total body muscle or body fluid after ghrelin treatments. The dotted line represents basal levels measured before beginning the experiment. All data are expressed as mean ± sem. a, Significant with respect to saline controls; b, significant with respect to ucp2^+/+ ghrelin.