Figure 1.

Effectiveness and specificity of S14 and S14R siRNA. Primary hepatocytes were treated with control (CTRL), S14, S14R, or both S14 and S14R siRNA as indicated for 6 h. Subsequently cells were incubated in high-glucose media for an additional 40 h. Total RNA was isolated and relative levels of S14 mRNA (A) or S14R mRNA (B) were measured by RT-PCR. Values are expressed relative to levels seen in cells treated with control siRNA and represent the means (±sd) of five samples. *, Groups that are statistically different from the control group (P ≤ 0.001).