Figure 2.

Rates of de novo lipogenesis in S14 and S14R siRNA-treated cells. Primary hepatocytes were cultured and transfected with siRNA as indicated in Materials and Methods. Forty hours after treatment with siRNA, ¹⁴C-acetate (1–5 μCi) was added and cells harvested 30 min later for measurement of de novo lipogenesis. The rate of lipogenesis was normalized to that observed in cells transfected with both S14 and S14R siRNA in each experiment. The data were pooled for seven independent experiments and are presented as mean (±sem) with n = 13, 12, 12, or 19 for control (CTRL), S14, S14R, and both S14 + S14R siRNA, respectively. Bars with different letters are significantly different from each other (P < 0.012).