Figure 4.

Both S14 and S14R are capable of fully rescuing de novo lipogenesis. Primary hepatocytes were treated with either control siRNA (CTRL) or both S14 and S14R siRNA (BOTH) for 6 h. Subsequently cells were treated with increasing amounts of adenovirus expressing either S14 or S14R for 2 h and then incubated for 40 h in high-glucose media. The rates of lipogenesis were measured as described in the legend to Fig. 2 and are expressed relative to that observed in control siRNA-treated cells. Values represent means (±sd) of four samples. In control experiments, transduction of hepatocytes with an adenovirus expressing green fluorescent protein did not affect the rates of lipogenesis.