Table 1. Primer sets and PCR conditions used in this study.
| Target mRNAa | Primer Sets | PCR cycle conditionsb denature/anneal/extend |
| IFN-γ | Fwd: 5′- TCAAGTGGCATAGATGTGGAAGAA -3′ Rev: 5′-TGGCTCTGCAGGATTTTCATG -3′ | 95°C, 30 s/60°C, 30 s/72°C, 30 s |
| TNF-α | Fwd: 5′- CATCTTCTCAAAATTCGAGTGACAA -3′ Rev: 5′- TGGGAGTAGACAAGGTACAACCC-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| KC | Fwd: 5′- TGCACCCAAACCGAAGTCAT-3′ Rev: 5′- TTGTCAGAAGCCAGCGTTCAC-3′ | 94°C, 30 s/57°C, 30 s/72°C, 45 s |
| MCP-1c | Fwd: 5′-TGCTACTCATTAACCAGCAAGAT -3′ Rev: 5′-TGCTTGAGGTGGTTGTGGAA -3′ | 94°C, 30 s/59°C, 15 s/72°C, 90 s +78°C, 5 s |
| iNOS | Fwd: 5′- TGGGAATGGAGACTGTCCCAG-3′ Rev: 5′- GGGATCTGAATGTGATGTTTG-3′ | 94°C, 30 s/60°C, 30 s/72°C, 30 s |
| mCRAMP | Fwd: 5′- CTTCAACCAGCAGTCCCTAGACA-3′ Rev: 5′- TCCAGGTCCAGGAGACGGTA-3′ | 94°C, 30 s/55°C, 30 s/72°C, 30 s |
| β-actin | Fwd: 5′-CAGCTTCTTTGCAGCTCCTT-3′ Rev: 5′-CTTCTCCATGTCGTCCCAGT-3′ | 94°C, 30 s/55–60°C, 30 s/72°C, 30 s |
| IL-17A | Fwd: 5′-GCTCCAGAAGGCCCTCAGA-3′ Rev: 5′-CTTTCCCTCCGCATTGACA-3′ | 94°C, 30 s/60°C, 30 s/72°C, 30 s |
| IL-17F | Fwd: 5′-TGCTACTGTTGATGTTGGGAC-3′ Rev: 5′-AATGCCCTGGTTTTGGTTGAA-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| IL-22 | Fwd: 5′-ACCTTTCCTGACCAAACTCA-3′ Rev: 5′-AGCTTCTTCTCGCTCAGACG-3′ | 94°C, 30 s/58°C, 30 s/72°C, 30 s |
| IL-23p19 | Fwd: 5′-TGGCTGTGCCTAGGAGTAGCA -3′ Rev: 5′-TTCATCCTCTTCTTCTCTTAGTAGATT -3′ | 94°C, 30 s/60°C, 30 s/72°C, 30 s |
| RegIII-γ | Fwd: 5′-TGCCTATGGCTCCTATTGCT-3′ Rev: 5′-CACTCCCATCCACCTCTGTT-3′ | 94°C, 30 s/58°C, 30 s/72°C, 30 s |
| Muc1 | Fwd: 5′-AGGAGGTTTCGGCAGGTAAT-3′ Rev: 5′-TCCTTCTGAGAGCCACCACT-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| Muc3/17 | Fwd: 5′-TGAGCAAAGGCAGTATCGTG-3′ Rev: 5′-GCCTCCTTCTTGCATGTCTC-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| Muc4 | Fwd: 5′-GAAAAGCGTGTTGCCTCTTC-3′ Rev: 5′-AGAGGGAAATGCCCTGATCT-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| Muc6 | Fwd: 5′-TGCATGCTCAATGGTATGGT-3′ Rev: 5′-TGTGGGCTCTGGAGAAGAGT-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| Muc13 | Fwd: 5′-TCTGGACTCTGGCCACTCTT-3′ Rev: 5′-GAGGACAGAGCCAGTCCAAG-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
| Muc19 | Fwd: 5′-ACTGGAACCACAGCCAAATC-3′ Rev: 5′-CTACGGCCTGTTTTTCGGTA-3′ | 94°C, 30 s/55°C, 30 s/72°C, 45 s |
aIFN-γ primers from ref. [94]; TNF-α primers, ref. [95]; MCP-1 primers, ref. [96]; mCRAMP primers, ref. [13]; iNOS primers, ref. [97]; KC primers, ref. [98]; IL-17A and IL-23p19 ref. [99]; IL-17F primers ref. [36]; and IL-22 primers ref. [100].
bAll PCR experiments had an initial denaturing step of 95°C for 3–5 mins before commencement of PCR cycling conditions.
cMCP1 primers after 40 cycles had an additional 2 steps of 94°C for 30 s, and 50°C for 30 s.