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. 2010 May 13;6(5):e1000894. doi: 10.1371/journal.ppat.1000894

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Soutourina et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The strains were grown in TSB medium with 2 mM cystine. A. Intracellular metabolite concentrations were estimated by HPLC for the ΔcymR mutant (SA17) and the parental SH1000 strain. The ΔcymR/SH1000 ratio is indicated. The complete data on metabolite concentrations are given in Table S3. B. H₂S production measurement was performed using the quantitative methylene blue method and a Na₂S standard curve. Representative results of lead-acetate paper assays are shown in the lower section. Strains SA6 (SH1000/pMK4), SA30 (ΔcymR/pMK4) and SA31 (ΔcymR/pDIA5780) were used. C. The pH of the medium was measured after an overnight culture (16 h) at 37°C. Strains SA6 (SH1000/pMK4), SA30 (ΔcymR/pMK4) and SA31 (ΔcymR/pDIA5780) were used. Results correspond to the mean values with standard deviations and are representative of at least two independent experiments.