Table 1. Stress response associated genes differentially expressed in the S. aureus ΔcymR mutant strain compared to SH1000.
| Gene namea (synonym) | Function/similarity | Transcriptome analysisb | qRT-PCRc | Differential expression under stress conditionsd | ||
| ΔcymR/SH1000 expression ratio | P value | ΔcymR/SH1000 expression ratio | ||||
| TSB+ Cys | TSB | |||||
| SA0366 ahpC* | alkyl hydroperoxide reductase, subunit C | 5.88 | <1.0E-16 | Nitrosative, nitrite, H2O2, diamide, paraquat | ||
| SA0365 ahpF* | alkyl hydroperoxide reductase, subunit F | 4.80 | <1.0E-16 | 8.52 | 1.73 | Nitrosative, nitrite |
| SA2481 | rhodanese family protein | 5.55 | <1.0E-16 | |||
| SA2345 copP | copper-ion-binding protein | 3.85 | <1.0E-16 | H2O2, nitrite | ||
| SA2344 copA | cation-transporting ATPase, E1-E2 family | 3.54 | <1.0E-16 | 12.48 | 1.35 | H2O2, nitrite |
| SA1709 ftnA | ferritin | 3.00 | <1.0E-16 | H2O2, nitrosative, nitrite | ||
| SA1941 dps* | general stress protein Dps | 2.83 | <1.0E-16 | 4.51 | 1.44 | H2O2, nitrosative, nitrite |
| SA1382 sodA* | superoxide dismutase | 2.75 | <1.0E-16 | 4.22 | 1.06 | Paraquat, nitrite |
| SA0128 sodM | superoxide dismutase | 1.52 | 1.16E-06 | 2.29 | 1.07 | Paraquat, nitrite |
| SA0719 trxB* | thioredoxin reductase | 1.70 | 3.07E-13 | 3.04 | 1.27 | Nitrite, diamide, paraquat |
| SA0992 trxA | thioredoxin | 1.62 | 3.54E-08 | 1.85 | Nitrite | |
| SA1979* | iron-compound ABC transporter component | 1.58 | 3.92E-05 | Nitrosative | ||
| SA0758 | thioredoxin, putative | 1.48 | 2.52E-06 | |||
| SA0755 | organic hydroperoxide resistance protein | 1.44 | 1.07E-09 | H2O2 | ||
| SA0231 | flavohemoprotein, putative | 1.42 | 5.29E-07 | H2O2, nitrosative, nitrite | ||
| SA1329 fur* | Fur regulator | 1.42 | 0.000036 | 2.37 | 1.42 | Nitrite |
| SA1678 perR* | PerR regulator | 1.42 | 0.0012 | 2.07 | 1.16 | |
| SA0531 proP | osmoprotectant proline transporter | 0.47 | <1.0E-16 | Nitrite | ||
a. The SA numbers (N315 strain) for S. aureus genes correspond to those of AureoList (http://genolist.pasteur.fr/AureoList/).
“*” indicates genes differentially expressed upon internalization of S. aureus in human epithelial cells [58].
b. The results obtained are representative of 8 hybridizations from 4 independent cultures in TSB medium with 2 mM cystine. The generated data sets were loaded into the GenoScript Database (http://genoscript.pasteur.fr)[25].
c. For qRT-PCR analysis, total RNA was extracted from S. aureus strains grown in TSB medium with or without 2 mM cystine. After reverse transcription, specific cDNAs was quantified by qRT-PCR using 16S rRNA gene for normalization.