Table 2. Strains and plasmids used in this study.
Strain or plasmid | Genotype or description | Reference or source |
Strain | ||
SH1000 | Functional rsbU+ derivative of 8325-4 wild-type strain | [70] |
S897 | 8325-4 perR::kan a | [34] |
S906 | 8325-4 fur::tet | [35] |
S1799 | SH1000 sodA::Tn917(ery) | [39] |
S739 | SH1000 sodM::tet | [39] |
SA17 | SH1000 ΔcymR | [25] |
SA6 | SH1000/pMK4 | [25] |
SA30 | SH1000 ΔcymR/pMK4 | [25] |
SA31 | SH1000 ΔcymR/pDIA5780 | [25] |
SA37 | SH1000 perR::kan | This study |
SA39 | SH1000 ΔcymR perR::kan | This study |
SA41 | SH1000 fur::tet | This study |
SA42 | SH1000 ΔcymR fur::tet | This study |
SA53 | SH1000 perR::kan fur::tet | This study |
SA61 | SH1000 ΔcymR perR::kan fur::tet | This study |
SA63 | SH1000 sodA::ery sodM::tet | This study |
Plasmids | ||
pMK4 | E. coli – S. aureus catr shuttle vector | [71] |
pDIA5780 | pMK4 derivative carrying the cymR gene for complementation | [25] |
akan, ery, tet and cat genes encode proteins leading to kanamycin, erythromycin, tetracycline and chloramphenicol resistance.