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. 2010 May 13;6(5):e1000888. doi: 10.1371/journal.ppat.1000888

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Tao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Physical map of the gene organization of the left end of Ф11. The relative positions of the probes used in the Northern blot shown in panel B and C are indicated. The scale indicates the lengths of the genes. (B) Northern blots containing 5 µg of total RNA per lane were probed with Φ11 int probe (left) and reprobed with cI probe (right). Lane 1: phage cured strain RN4220; lane 2: Φ11 lysogen RN4220Φ11; lane 3: sigH mutant strain RN4220ΔsigHΦ11; lane 4: complementary strain RN4220ΔsigHcΦ11; lane 5: RN4220Φ11Δint. Ethidium bromide stained 16S rRNA patterns are shown as an indication of RNA loading. RL6000 (Takara) RNA marker (lane M) was used to estimate the molecular weight of the fragments. (C) Same RNA samples were probed with Φ11 xis probe at low molecular size. Lane 1: RN4220; lane 2: RN4220Φ11; lane 3: RN4220ΔsigHΦ11; lane 4: RN4220ΔsigHcΦ11; lane 5: RN4220Φ11Δint. RL1000 (Takara) RNA marker (lane M) was used to estimate the molecular weight of the fragments.