Figure 8. SigH plays a regulatory role on prophage excision and integration via integrase.
Primer pairs from two sides of attB (check-F and check-R) were used for determination of the ratio of the excised phage genome. Primer pair with one primer outside the att site and the other primer inside the prophage (check-R and check-IN) was used for determination of the ratio of the integrated prophage. SigH deletion resulted in an increase in excised phage genomes. The ratios of excised prophage genomes were similar in WT and the complementation (n = 4 samples/group, *p<0.05).
