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. 2010 May 13;5(5):e10636. doi: 10.1371/journal.pone.0010636

Figure 4. CD300a+ CD4 T cells are more polyfunctional than CD300a CD4 T cells.

Figure 4

(A) Purified CD4 T cells were stimulated with PMA and ionomycin for 4–5 h. Then, cells were stained for cell surface expression of CD300a and production of IFN-γ and TNF-α and/or IL-2. The percentage of double and triple producers in the CD300a+ and CD300a cells is shown. Each symbol represents a different donor. (B) Graphic representation of the average ± SEM of the MFI of IFN-γ, IL-2 or TNF-α for the triple producers shown in panel A. (C) Purified CD4 T cells were stimulated with PMA and ionomycin for 4–5 h. Then, cells were stained for cell surface expression of CD300a and the production of the indicated cytokines. The percentage of triple cytokine producers in the CD300a+ and CD300a subsets is shown. Each symbol is a different donor.