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. 2010 May 13;5(5):e10636. doi: 10.1371/journal.pone.0010636

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Purified CD4 T cells were sorted into CD45RO⁺CD300a⁺ (black bars) and CD45RO⁺CD300a⁻ (white bars). RNA was extracted and the levels of mRNAs were determined by real-time PCR. Graphs represent the average ± SEM. AU: arbitrary units. Data are from 5–6 donors. These data correspond to the untreated (none) samples in Figures 5B and 6A. (B) Purified CD4 T cells were sorted into CD45RO⁺CD300a⁺ and CD45RO⁺CD300a⁻ and then stimulated overnight with plate bound anti-CD3 or anti-CD3 plus anti-CD28 mAb. RNA was extracted from cells and specific cytokine mRNAs were quantified by real-time PCR. (C) Supernatants from the cultures were harvested and the levels of cytokines were measured.