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. 2010 May 13;5(5):e10627. doi: 10.1371/journal.pone.0010627

Figure 2. Spontaneous and self-seeded fibrillization of SOD1.

Figure 2

Spontaneous and self-seeded reactions for (A) wtSOD1, (B) G93A, (C) G37R, (D) A4V, and (E) G85R. All SOD1 variants, except G85R were incubated at 37°C, at pH4.0 in mild denaturing condition (0.5M GdnHCl). Fibrillization of G85R was performed at pH5.0 with no denaturant. Fibril formation is reported as % maximum ThT relative fluorescence units (RFU) shown as a function of time (hrs). Seeded reactions were spiked with 1% preformed fibrils (v/v). Data shown are average values from 6–10 replicates (± SEM) pooled from 2–3 independent assays. Seeded fibrillization reactions consistently shortened the lag times by an average of 82% (except for G85R) compared to spontaneous reactions under similar conditions.