Figure 6. The effect of protein stability on the lag time of SOD1 fibrillization for spontaneous and self-seeded reactions.

The plot shows the correlation of SOD1 stability, in the metallated and intra-subunit intact form, (expressed as melting temperature, Tm (°C)) to lag time (hrs). Destabilisation of SOD1 correlated with shorter lag times for fibrillisation in spontaneous reactions, whereas reactions initiated with pre-formed fibrils had lag times that were independent of the original native fold stability. (Tm data for G85R not available, thus it was not possible to include the G85R fibrillization lag time and propagation rate in the graphs.)